Title: Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry
Author: Ribeiro, Lígia N. M.; Franz-montan, Michelle; Alcântara, Ana C. S.; Breitkreitz, Márcia C.; Castro, Simone R.; Guilherme, Viviane A.; Muniz, Bruno V.; Rodrigues Da Silva, Gustavo H.; De Paula, Eneida
Is part of: Scientific Reports, v. 10, p. 11341 -
Citation: Ribeiro, Lígia N. M.; Franz-montan, Michelle; Alcântara, Ana C. S.; Breitkreitz, Márcia C.; Castro, Simone R.; Guilherme, Viviane A.; Muniz, Bruno V.; Rodrigues Da Silva, Gustavo H.; De Paula, Eneida; Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry. Scientific Reports, v.10, p. 11341-, 2020
Abstract: Topical anesthetics are widely applied in order to relieve the discomfort and anxiety caused by needle insertion and other painful superficial interventions at the oral cavity. So far, there are no commercially available effective topical anesthetic formulations for that purpose, and the most of developments are related to hydrophilic and low mucoadhesive forms. Therefore, we have prepared different hybrid nanofilms composed of biopolymer matrices (chitosan, pectin, and chitosan-pectin) blended with nanostructured lipid carriers (NLC) loading the eutectic mixture of 5% lidocaine-prilocaine (LDC-PLC), in order to fulfill this gap in the market. These dual systems were processed as hybrid nanofilms by the solvent/casting method, and its mucoadhesive, structural and mechanical properties were detailed. The most appropriate hybrid nanofilm combined the advantages of both pectin (PCT) and NLC components. The resultant material presented sustained LDC-PLC release profile for more than 8 h; permeation across porcine buccal mucosa almost twice higher than control and non-cytotoxicity against 3T3 and HACAT cell lines. Then, the in vivo efficacy of PCT/NLC formulation was compared to biopolymer film and commercial drug, exhibiting the longest-lasting anesthetic effect (>7 h), assessed by tail flick test in mice. These pectin-based hybrid nanofilms open perspectives for clinical trials and applications beyond Dentistry.
Keywords: drug delivery; hybrid hydrogel; lipid nanoparticles; local anesthetics;
Subjects: CIENCIAS_BIOLOGICAS; Farmacologia Bioquímica e Molecular; CIENCIAS_BIOLOGICAS; Biofísica; Biofísica de Processos e Sistemas;
Funding: The authors thank FundacAo de Amparo a Pesquisa do Estado de SAo Paulo (FAPESP 14/25372-0; 14/14457-5), INCT-Bioanalitica (FAPESP/INCT 14/50867-3; CNPq/INCT 465389/2014-7), FAPEMA (IECT-Biotecnologia; 00961/18), CAPES (88882.314720/2013-01) and CNPq (425730/2018-2) for financial support, and Cristalia Chem. Pharm. Ind. for kindly providing lidocaine and prilocaine.