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Title: Influence of chitosan-tripolyphosphate nanoparticles on thermosensitive polymeric hydrogels: structural organization, drug release mechanisms and cytotoxicity Author: Freitas Mariano, Kelli C.; Lombello, Christiane B.; De Lima, Renata; Fraceto, Leonardo F.; De Araujo, Daniele R.; Monteiro Do Nascimento, Mônica H.; Querobino, Samyr M.; Ramos Campos, Estefânia V.; De Oliveira, Jhones L.; Yokaichiya, Fabiano; Franco, Margareth K.K.D.; Alberto-silva, Carlos; De Paula, Eneida Year: 2019 Is part of: International Journal of Polymeric Materials and Polymeric Biomaterials, v. 1, p. 1 - 12 DOI: https://doi.org/10.1080/00914037.2019.1596909 Citation: Freitas Mariano, Kelli C.; Lombello, Christiane B.; De Lima, Renata; Fraceto, Leonardo F.; De Araujo, Daniele R.; Monteiro Do Nascimento, Mônica H.; Querobino, Samyr M.; Ramos Campos, Estefânia V.; De Oliveira, Jhones L.; Yokaichiya, Fabiano; Franco, Margareth K.K.D.; Alberto-silva, Carlos; De Paula, Eneida; Influence of chitosan-tripolyphosphate nanoparticles on thermosensitive polymeric hydrogels: structural organization, drug release mechanisms and cytotoxicity. International Journal of Polymeric Materials and Polymeric Biomaterials, v.1, p. 1-12, 2019 Abstract: Chitosan-tripolyphosphate (CS-TPP) nanoparticles containing naproxen (NPX) were dispersed in poloxamer (PL) as unique (PL407) or binary (PL407-PL403) systems. Nanoparticles presented diameter of similar to 250 nm and zeta potential of similar to 35 mV with drug loading and encapsulation efficiency of 98.4 +/- 0.3% and 36.9 +/- 0.12%, respectively. NPX-CS-TPP shifted the sol-gel transition and micellization temperatures. PL407-PL403 systems presented G ' > G '' compared to PL407. SAXS patterns revealed transitions from lamellar to hexagonal phase organizations with low drug release rates, in the presence of CS-TPP nanoparticles. NPX-CS-TPP-PL407 induced lower cytotoxicity compared to PL407-PL403 in fibroblasts and osteoblasts, making them promising systems for intra-articular delivery. Subjects: CIENCIAS_BIOLOGICAS; Farmacologia Bioquímica e Molecular; Funding: This research work was supported by Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Amparoa Pesquisa do Estado de Sao Paulo [FAPESP 2014/26200-9, 2014/14457-5, 2014/12653-1] and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [CNPq 309207/2016-9, 402838/2016-5]. Authors are also grateful to the Brazilian Synchrotron Light Laboratory for SAXS facilities (SAXS 1 beamline) and UFABC Multiuser Central Facilities (CEM-UFABC). |
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