Article

Download article 		Title: In vitro sensitivity of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Brazilian isolates to meglumine antimoniate and amphotericin B
Author: Zauli-nascimento, R. C.; Miguel, D. C.; Yokoyama-yasunaka, J. K. U.; Pereira, Lia; Oliveira, M.; Ribeiro-dias, F.; Dorta, Ml; Uliana, S. R. B.
Year: 2010
Is part of: TM & IH. Tropical medicine and international health (Print), v. 15, p. 68 - 76
DOI: https://doi.org/10.1111/j.1365-3156.2009.02414.x

Citation: Zauli-nascimento, R. C.; Miguel, D. C.; Yokoyama-yasunaka, J. K. U.; Pereira, Lia; Oliveira, M.; Ribeiro-dias, F.; Dorta, Ml; Uliana, S. R. B.; In vitro sensitivity of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Brazilian isolates to meglumine antimoniate and amphotericin B. TM & IH. Tropical medicine and international health (Print), v.15, p. 68-76, 2010

Abstract: Resistance of Leishmania parasites to specific chemotherapy has become a well-documented problem in the Indian subcontinent in recent years but only a few studies have focused on the susceptibility of American Leishmania isolates. Our susceptibility assays to meglumine antimoniate were performed against intracellular amastigotes after standardizing an in vitro model of macrophage infection appropriate for Leishmania (Viannia) braziliensis isolates. For the determination of promastigote susceptibility to amphotericin B, we developed a simplified MTT-test. The sensitivity in vitro to meglumine antimoniate and amphotericin B of 13 isolates obtained from Brazilian patients was determined. L. (V.) braziliensis isolates were more susceptible to meglumine antimoniate than Leishmania (Leishmania) amazonensis. EC50, EC90 and activity indexes (calculated over the sensitivity of reference strains), suggested that all isolates tested were susceptible in vitro to meglumine antimoniate, and did not show association with the clinical outcomes. Isolates were also uniformly susceptible in vitro to amphotericin B.



Funding: We thank Dr Lucile M. Floeter-Winter for assistance with the isolates' identification and Dr. Jeffrey J. Shaw for helpful comments and discussions. This work has received financial support from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) and Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP). R.C.Z-N. and D.C.M. were recipients of CNPq and FAPESP fellowships, respectively. 		Financed by: