Title: Development and characterization of microsatellite loci for Tabebuia cassinoides (Bignoniaceae)
Author: Pretti, V.Q.; Françoso, E.; Sforça, D.A.; Pinheiro, F.; Meyer, D.; Lohmann, L.G.
Is part of: Genetics and Molecular Research, v. 13, p. 5601 - 5605
Citation: Pretti, V.Q.; Françoso, E.; Sforça, D.A.; Pinheiro, F.; Meyer, D.; Lohmann, L.G.; Development and characterization of microsatellite loci for Tabebuia cassinoides (Bignoniaceae). Genetics and Molecular Research, v.13, p. 5601-5605, 2014
Abstract: Tabebuia cassinoides (Lam.) DC., popularly known as caxeta, is a tree species that belongs to the plant family Bignoniaceae. This species is endemic to the Brazilian Atlantic Forest and is widely exploited commercially. To date, little is known about its genetic structure, preventing the establishment of adequate management plans for this taxon. The objective of this study was to construct a microsatellite-enriched genomic library for T. cassinoides to select polymorphic loci, and standardize polymerase chain reaction amplification conditions. Of the 15 loci examined, 5 were polymorphic. The number of alleles per locus ranged from 2 to 8, with a mean of 4.4. The microsatellite loci described here represent the basis for detailed population genetic studies of this species, which will greatly contribute for the development of better conservation strategies for this taxon.
Funding: We would like to thank Alexandre Rizzo Zuntini for assistance during fieldwork and Drs. Anette P. de Souza, Ana Cristina Bondioli, Daniela Calcagnotto, and Riviane Garcez for assistance during the preparation of the genomic library. We also thank CNPq for a Ph.D. scholarship to V.Q. Pretti (Grant #142040/2009-6) and a Pq-1D grant to L.G. Lohmann (Grant #305102/2010-9), FAPESP for a Ph.D. scholarship to E. Francoso (Grant #2010/20548-2), and CAPES/PROEX for additional funding.