Article

Download article 		Title: Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
Author: Novello, M.; Rodrigues, J. F.; Pinheiro, F.; Oliveira, G. C. X.; Veasey, E. A.; Koehler, Samantha
Year: 2013
Is part of: Genetics and Molecular Research, v. 12, p. 3274 - 3278
DOI: https://doi.org/10.4238/2013.September.3.3

Citation: Novello, M.; Rodrigues, J. F.; Pinheiro, F.; Oliveira, G. C. X.; Veasey, E. A.; Koehler, Samantha; Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest. Genetics and Molecular Research, v.12, p. 3274-3278, 2013

Abstract: Microsatellite markers were developed for the endangered Brazilian orchid species Cattleya coccinea to describe its genetic diversity and structure and to support conservation studies. Nine microsatellite loci were isolated and characterized using an enriched genomic library. All loci are polymorphic at least in the 2 populations sampled, except for loci Cac05 and Cac09 for the Petropolis population. The mean number of alleles per locus was 8.8 between populations. The mean values of the observed and expected heterozygosities were 0.541 (ranging from 0 to 1) and 0.639 (ranging from 0 to 0.9), respectively. Cross-amplifications were performed in 7 additional Epidendroideae species, and at least 2 loci were successful in 3 additional Cattleya species, Epidendrum secundum, and Brasiliorchis gracilis. All markers described herein will be useful in further studies evaluating the genetic diversity, population dynamics, and conservation genetics of C. coccinea and related species.



Funding: Research supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (grants #2006/55121-3 and #2011/18532-3). The authors thank A. P. Souza, M. Oliveira, and C. Grando for technical support, and E. Catharino and Instituto de Botanica de Sao Paulo for providing specimens for study. 		Financed by: