Article

Download article 		Title: Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
Author: Pinheiro, Fábio; Palma-silva, Clarisse; Barros, Fábio De; Cozzolino, Salvatore
Year: 2009
Is part of: Genetics and Molecular Biology (Impresso), v. 32, p. 337 - 339
DOI: https://doi.org/10.1590/S1415-47572009005000037

Citation: Pinheiro, Fábio; Palma-silva, Clarisse; Barros, Fábio De; Cozzolino, Salvatore; Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum. Genetics and Molecular Biology (Impresso), v.32, p. 337-339, 2009

Abstract: In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity ( average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.



Funding: This work was supported by grants from FAPESP (06/54189-3) and CNPq (471929/2006-9) to FP and FB, and by awards from CNPq and CAPES to FP and FB. 		Financed by: