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Title: Hyperbaric oxygen affects endothelial progenitor cells proliferation in vitro Author: Benincasa, Julia Carnaz; De Filho, Luiz Henrique Freitas; Carneiro, Giane Daniela; Sielski, Micheli Severo; Giorgio, Selma; Werneck, Claudio Chrysostomo; Vicente, Cristina Pontes Year: 2018 Is part of: CELL BIOLOGY INTERNATIONAL, v. 31, p. 10 - DOI: https://doi.org/10.1002/cbin.11070 Citation: Benincasa, Julia Carnaz; De Filho, Luiz Henrique Freitas; Carneiro, Giane Daniela; Sielski, Micheli Severo; Giorgio, Selma; Werneck, Claudio Chrysostomo; Vicente, Cristina Pontes; Hyperbaric oxygen affects endothelial progenitor cells proliferation in vitro. CELL BIOLOGY INTERNATIONAL, v.31, p. 10-, 2018 Abstract: Hyperbaric oxygen is a clinical treatment that contributes to wound healing by increasing fibroblasts proliferation, collagen synthesis, and production of growth factors, inducing angiogenesis and inhibiting antimicrobial activity. It also has been shown that hyperbaric oxygen treatment (HBO), through the activation of nitric oxide synthase promotes an increase in the nitric oxide levels that may improve endothelial progenitor cells (EPC) mobilization from bone marrow to the peripheral blood and stimulates the vessel healing process. However, cellular mechanisms involved in cell proliferation and activation of EPC after HBO treatment remain unknown. Therefore, the present work aimed to analyze the effect of HBO on the proliferation of pre-treated bone marrow-derived EPC with TNF-alpha. Also, we investigated the expression of ICAM and eNOS by immunochemistry, the production of reactive species of oxygen and performed an in vitro wound healing. Although 1h of HBO treatment did not alter the rate of in vitro wound closure or cell proliferation, it increased eNOS expression and decreased ICAM expression and reactive oxygen species production in cells pre-treated with TNF-alpha. These results indicate that HBO can decrease the inflammatory response in endothelial cells mediated by TNF-alpha, and thus, promote vascular recovery after injury. Funding: J.C.B. elaborated the project, isolated the cells and performed all the assays with the cells. L.H.F.J and G.D.C helped with cell isolation and culture, M.S.S helped with histological assays, S.G helped with the assays using HBO. C.C.W helped with the cell culture and reviewed the article; C.P.V was responsible for the overall project helped to write the article and with the wound-healing assay. This work was supported by grants from Coordination for the Improvement of Higher Education Personnel (CAPES) to J.C.B, L.H.F.J and G.D.C. C.P.V and S.G were supported by Sao Paulo Research Foundation (FAPESP) (2012/23640) and (2015/23767-0) respectively. C.C.W and M.S.S. to National Council for Scientific and Technological Development (CNPq). C.C.W (308368/2016-9). |
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