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Title: HSP70 of Leishmania amazonensis alters resistance to different stresses and mitochondrial bioenergetics
Author: Codonho, Bárbara Santoni; Costa, Solange Dos Santos; Peloso, Eduardo De Figueiredo; Joazeiro, Paulo Pinto; Gadelha, Fernanda Ramos; Giorgio, Selma
Year: 2016
Is part of: Memórias do Instituto Oswaldo Cruz (Online), v. 111, p. 460 - 468
DOI: https://doi.org/10.1590/0074-02760160087

Citation: Codonho, Bárbara Santoni; Costa, Solange Dos Santos; Peloso, Eduardo De Figueiredo; Joazeiro, Paulo Pinto; Gadelha, Fernanda Ramos; Giorgio, Selma; HSP70 of Leishmania amazonensis alters resistance to different stresses and mitochondrial bioenergetics. Memórias do Instituto Oswaldo Cruz (Online), v.111, p. 460-468, 2016

Abstract: The 70 kDa heat shock protein (HSP70) is a molecular chaperone that assists the parasite Leishmania in returning to homeostasis after being subjected to different types of stress during its life cycle. In the present study, we evaluated the effects of HSP70 transfection of L. amazonensis promastigotes (pTEX-HSP70) in terms of morphology, resistance, infectivity and mitochondrial bioenergetics. The pTEX-HSP70 promastigotes showed no ultrastructural morphological changes compared to control parasites. Interestingly, the pTEX-HSP70 promastigotes are resistant to heat shock, H2O2-induced oxidative stress and hyperbaric environments. Regarding the bioenergetics parameters, the pTEX-HSP70 parasites had higher respiratory rates and released less H2O2 than the control parasites. Nevertheless, the infectivity capacity of the parasites did not change, as verified by the infection of murine peritoneal macrophages and human macrophages, as well as the infection of BALB/c mice. Together, these results indicate that the overexpression of HSP70 protects L. amazonensis from stress, but does not interfere with its infective capacity.



Funding: FAPESP, CNPq, CAPES.
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