Title: Comparative in vitro toxicity of a graphene oxide-silver nanocomposite and the pristine counterparts toward macrophages
Author: De Luna, Luis Augusto Visani; De Moraes, Ana Carolina Mazarin; Consonni, Sílvio Roberto; Pereira, Catarinie Diniz; Cadore, Solange; Giorgio, Selma; Alves, Oswaldo Luiz
Is part of: Journal of Nanobiotechnology, v. 14, p. 12 -
Citation: De Luna, Luis Augusto Visani; De Moraes, Ana Carolina Mazarin; Consonni, Sílvio Roberto; Pereira, Catarinie Diniz; Cadore, Solange; Giorgio, Selma; Alves, Oswaldo Luiz; Comparative in vitro toxicity of a graphene oxide-silver nanocomposite and the pristine counterparts toward macrophages. Journal of Nanobiotechnology, v.14, p. 12-, 2016
Abstract: Background: Graphene oxide (GO) is a highly oxidized graphene form with oxygen functional groups on its surface. GO is an excellent platform to support and stabilize silver nanoparticles (AgNP), which gives rise to the graphene oxide-silver nanoparticle (GOAg) nanocomposite. Understanding how this nanocomposite interacts with cells is a toxicological challenge of great importance for future biomedical applications, and macrophage cells can provide information concerning the biocompatibility of these nanomaterials. The cytotoxicity of the GOAg nanocomposite, pristine GO, and pristine AgNP was compared toward two representative murine macrophages: a tumoral lineage (J774) and peritoneal macrophages collected from Balb/c mouse. The production of reactive oxygen species (ROS) by J774 macrophages was also monitored. We investigated the internalization of nanomaterials by transmission electron microscopy (TEM). The quantification of internalized silver was carried out by inductively coupled plasma mass spectrometry (ICP-MS). Nanomaterial stability in the cell media was investigated overtime by visual observation, inductively coupled plasma optical emission spectrometry (ICP OES), and dynamic light scattering (DLS).
Funding: The authors thank the National Council for Technological and Scientific Development (CNPq) for the PhD student scholarship (140560/2014-9) and the financial support. The authors also acknowledge Dr. Daniel Ruiz Abanades for suggestions, Renata Magueta for support in the ICP OES analysis, Douglas Soares for AFM imaging, and the Institute of Food Technology (ITAL) for support in the ICP-MS analysis. We thank the Laboratory of Synthesis of Nanostructures and Interaction with Biosystems (NanoBioss), the Brazilian Nanotoxicology Network-Cigenanotox and the National Institute of Science, Technology and Innovation in Complex Functional Materials (INOMAT/INCT) for general support.