Title: Effects of hyperbaric oxygen on Pseudomonas aeruginosa susceptibility to imipenem and macrophages
Author: Lima, F. L.; Joazeiro, P. P.; Lancelloti, M.; Holanda, L.; Linares, E.; Augusto, O.; Brocchi, M.; Giorgio, S.
Is part of: Future Microbiology (Print), v. 10, p. 179 - 189
Citation: Lima, F. L.; Joazeiro, P. P.; Lancelloti, M.; Holanda, L.; Linares, E.; Augusto, O.; Brocchi, M.; Giorgio, S.; Effects of hyperbaric oxygen on Pseudomonas aeruginosa susceptibility to imipenem and macrophages. Future Microbiology (Print), v.10, p. 179-189, 2015
Abstract: Background: The seriousness to treat burn wounds infected with Pseudomonas aeruginosa led us to examine whether the effect of the carbapenem antibiotic imipenem is enhanced by hyperbaric oxygen (HBO). Materials & methods: The effects of HBO (100% O-2, 3 ATA, 5 h) in combination with imipenen on bacterial counts of six isolates of P. aeruginosa and bacterial ultrastructure were investigated. Infected macrophages were exposed to HBO (100% O-2, 3 ATA, 90 min) and the production of reactive oxygen species monitored. Results: HBO enhanced the effects of imipenen. HBO increased superoxide anion production by macrophages and likely kills bacteria by oxidative mechanisms. Conclusion: HBO in combination with imipenem
Funding: This work was supported by grants of Fundacao de Amparo a Pesquisa do Estado de Sao Paulo and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil. EL and OA are members of the CEPID Redoxoma (FAPESP # 2013/07937-8). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.