Title: Digging deeper in the proteome of different regions from schizophrenia brains
Author: Reis-de-oliveira, G.; Zuccoli, G.S.; Fioramonte, M.; Schimitt, A.; Falkai, P.; Almeida, V.; Martins-de-souza, D.
Is part of: Journal of Proteomics, v. 223, p. 103814 - 103814
Citation: Reis-de-oliveira, G.; Zuccoli, G.S.; Fioramonte, M.; Schimitt, A.; Falkai, P.; Almeida, V.; Martins-de-souza, D.; Digging deeper in the proteome of different regions from schizophrenia brains. Journal of Proteomics, v.223, p. 103814-103814, 2020
Abstract: Schizophrenia is a psychiatric disorder that affects 21 million people worldwide. Despite several studies having been shown that some brain regions may play a critical role in the pathophysiology o f schizophrenia, the mo lecular basis to explain this diversity is still lacking. The cerebellum (CER), caudate nucleus (CAU), and posterior cingulate cortex (PCC) are areas associated with negative and cognitive symptoms in schizophrenia. In this study, w e performed shotgun proteomics o f the aforementioned brain regions, collected postmortem from pa tients with schizophrenia and compared with the mentally healthy group. In addition, w e performed a proteomic analysis o f nuclear and mitochondrial fractions of these same regions. Our results presented 106, 727 and 135 differentially regulated proteins in the CAU, PCC, and CER, respectively. Pathway enrichment analysis revealed dysfunctions associated with synaptic processes in the CAU, transport in the CER, and in energy metabolism in the PCC. In all brain areas, w e found that proteins related to oligodendrocytes and the metabolic processes were dysregulated in schizophrenia.
Funding: GRO, GSZ, MF, VA, and DMS would like to thank Sao Paulo Resarch Foundation (FAPESP) for funding (under grant numbers 2014/10068-4, 2018/01410-1, 2018/03673-0, 2017/25588-1, 2019/00098-7, 2016/18715-4, 2017/18242-1, 2018/14666-4, 2014). Authors thank Bradley Smith for proofreading (2016/07948-8).