Article

Download article 		Title: Evidence of macrophage modulation in the mouse pubic symphysis remodeling during the end of first pregnancy and postpartum
Author: Castelucci, B. G.; Consonni, S. R.; Pereira, A. H. M.; Fioramonte, M.; Carazzolle, M. F.; De Oliveira, P. S. L.; Franchini, K. G.; Kobarg, J.; Martins-de-souza, D.; Joazeiro, P. P.
Year: 2020
Is part of: Scientific Reports, v. 10, p. 12403 -
DOI: https://doi.org/10.1038/s41598-020-68676-x

Citation: Castelucci, B. G.; Consonni, S. R.; Pereira, A. H. M.; Fioramonte, M.; Carazzolle, M. F.; De Oliveira, P. S. L.; Franchini, K. G.; Kobarg, J.; Martins-de-souza, D.; Joazeiro, P. P.; Evidence of macrophage modulation in the mouse pubic symphysis remodeling during the end of first pregnancy and postpartum. Scientific Reports, v.10, p. 12403-, 2020

Abstract: In mouse pregnancy, pubic symphysis (PS) remodels into an elastic interpubic ligament (IpL) in a temporally regulated process to provide safe delivery. It restores at postpartum to assure reproductive tract homeostasis. Recently, macrophage localization in the IpL and dynamic changes in the expression of inflammatory mediators observed from the end of pregnancy (D18, D19) to early days postpartum (1dpp, 3dpp) highlighted the necessity of the identification of the key molecules involved in innate immune processes in PS remodeling. Therefore, this study uses morphological and high-sensitivity molecular techniques to identify both macrophage association with extracellular matrix (ECM) remodeling and the immunological processes involved in PS changes from D18 to 3dpp. Results showed macrophage association with active gelatinases and ECM components and 25 differentially expressed genes (DEGs) related to macrophage activities in interpubic tissues from D18 to 3dpp. Additionally, microarray and proteomic analysis showed a significant association of interpubic tissue DEGs with complement system activation and differentially expressed proteins (DEPs) with phagocytosis, highlighting the involvement of macrophage-related activities in mouse PS remodeling. Therefore, the findings suggest that PS ECM remodeling is associated with evidence of macrophage modulation that ensures both IpL relaxation and fast PS recovery postpartum for first labor.



Funding: This study was supported by FAPESP (2015/23616-2 and 2017/03489-1) and by CNPq (140714/2016-2, 302208/2017-8 and 421841/2018-4) grants. We thank the National Institute of Science and Technology on Photonics Applied to Cell Biology (INFABIC) and Electron Microscopy Laboratory at UNICAMP for access to equipment and assistance; INFABIC is co-funded by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) (08/57906-3) and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) (573913/2008-0). The authors also thank Maria Eugenia Ribeiro de Camargo for excellent technical assistance and Dr. Cristina Pontes Vicente for kindly providing VEGFR2 antibody aliquot. 		Financed by: