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Download article 		Title: Outer Membrane Vesicles from Neisseria Meningitidis (Proteossome) Used for Nanostructured Zika Virus Vaccine Production
Author: Martins, Paula; Melo, Carlos Fernando Odir Rodrigues; Morishita, Karen Noda; Catharino, Rodrigo Ramos; Arns, Clarice Weis; Lancellotti, Marcelo; Machado, Daisy; Theizen, Thais Holtz; Guarnieri, João Paulo Oliveira; Bernardes, Bruno Gaia; Gomide, Gabriel Piccirillo; Corat, Marcus Alexandre Finzi; Abbehausen, Camilla; Módena, José Luiz Proença
Year: 2018
Is part of: Scientific Reports, v. 8, p. 1 -
DOI: https://doi.org/10.1038/s41598-018-26508-z

Citation: Martins, Paula; Melo, Carlos Fernando Odir Rodrigues; Morishita, Karen Noda; Catharino, Rodrigo Ramos; Arns, Clarice Weis; Lancellotti, Marcelo; Machado, Daisy; Theizen, Thais Holtz; Guarnieri, João Paulo Oliveira; Bernardes, Bruno Gaia; Gomide, Gabriel Piccirillo; Corat, Marcus Alexandre Finzi; Abbehausen, Camilla; Módena, José Luiz Proença; Outer Membrane Vesicles from Neisseria Meningitidis (Proteossome) Used for Nanostructured Zika Virus Vaccine Production. Scientific Reports, v.8, p. 1-, 2018

Abstract: The increase of Zika virus (ZIKV) infections in Brazil in the last two years leaves a prophylactic measures on alert for this new and emerging pathogen. Concerning of our positive experience, we developed a new prototype using Neisseria meningitidis outer membrane vesicles (OMV) on ZIKV cell growth in a fusion of OMV in the envelope of virus particles. The fusion of nanoparticles resulting from outer membrane vesicles of N. meningitidis with infected C6/36 cells line were analyzed by Nano tracking analysis (NTA), zeta potential, differential light scattering (DLS), scan and scanning transmission eletronic microscopy (SEM and STEM) and high resolution mass spectometry (HRMS) for nanostructure characterization. Also, the vaccination effects were viewed by immune response in mice protocols immunization (ELISA and inflammatory chemokines) confirmed by Zika virus soroneutralization test. The results of immunizations in mice showed that antibody production had a titer greater than 1: 160 as compared to unvaccinated mice. The immune response of the adjuvant and non-adjuvant formulation activated the cellular immune response TH1 and TH2. In addition, the serum neutralization was able to prevent infection of virus particles in the glial tumor cell model (M059J). This research shows efficient strategies without recombinant technology or DNA vaccines.



Funding: Thanks for Beatriz Jacyntho Siqueira, Prof. Dr. Daniel F. Kawano for the moral support for develop this article. Special acknowledgements for Prof. Dr. Glaucia Maria Pastore from the initiative for ZIKA Task Force in UNICAMP. Thanks for Prof. Dr. Eneida de Paula for use of NTA equipment (FAPESP 2014/14457-5), Carlos Fernando Odir Rodrigues de Melo (fellowship FAPESP 2016/17066-2), Prof. Dr. Camila Abbehausen in the acquisition and STEM images interpretation (FAPESP 2017/12719-0). Special thanks for the technician Hugo Campos Loureiro for disponibility in perfomed in short time the electronic microscopy images. 		Financed by: