Article

Download article 		Title: Cilostazol attenuates oxidative stress and apoptosis in the quadriceps muscle of the dystrophic mouse experimental model.
Author: Hermes, Túlio De Almeida; Mâncio, Rafael Dias; Mizobutti, Daniela Sayuri; Macedo, Aline Barbosa; Kido, Larissa Akemi; Cagnon Quitete, Valéria Helena Alves; Minatel, Elaine
Year: 2022
Is part of: INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, v. 00, p. 1 - 10
DOI: https://doi.org/10.1111/iep.12461

Citation: Hermes, Túlio De Almeida; Mâncio, Rafael Dias; Mizobutti, Daniela Sayuri; Macedo, Aline Barbosa; Kido, Larissa Akemi; Cagnon Quitete, Valéria Helena Alves; Minatel, Elaine; Cilostazol attenuates oxidative stress and apoptosis in the quadriceps muscle of the dystrophic mouse experimental model.. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, v.00, p. 1-10, 2022

Abstract: Duchenne muscular dystrophy (DMD) is the most severe and frequent form of muscular dystrophy. The mdx mouse is one of the most widely used experimental models to understand aspects of the biology of dystrophic skeletal muscles and the mechanisms of DMD. Oxidative stress and apoptosis are present in early stages of the disease in mdx mice. The high production of reactive oxygen species (ROS) causes activation of apoptotic death regulatory proteins due to DNA damage and breakdown of nuclear and mitochondrial membranes. The quadriceps (QUA) muscle of the mdx mouse is a good tool to study oxidative events. Previous studies have demonstrated that cilostazol exerts an anti-oxidant effect by decreasing the production of reactive oxygen species (ROS). The present study aimed to evaluate the ability of cilostazol to modulate oxidative stress and apoptosis in the QUA muscle of mdx mice. Fourteen-day-old mdx mice received cilostazol or saline for 14 days. C57BL/10 mice were used as a control. In the QUA muscle of mdx mice, cilostazol treatment decreased ROS production (-74%), the number of lipofuscin granules (-47%), lipid peroxidation (-11%), and the number of apoptotic cells (-66%). Thus cilostazol showed anti-oxidant and anti-apoptotic action in the QUA muscle of mdx mice.



Funding: Conselho Nacional de Desenvolvimento Cientifico e Tecnologico; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Grant/Award Number: 11/02474-4 		Financed by: